RESUMO
Leaf fractions of Garcinia brasiliensis were evaluated concerning their antioxidant, antimicrobial, anti-inflammatory and cytotoxic properties, and the most active fraction was then fully characterized regarding its phenolic composition using HPLC-DAD-ESI/MSn. The ethyl acetate fraction from partitioning of the methanolic leaf extract revealed a strong antioxidant activity that was comparable to Trolox, the positive control. This fraction was also able to show a significant antimicrobial activity against Gram-positive and Gram-negative bacteria and the fungus Candida albicans. However, the dichloromethane fraction was found to present the highest anti-inflammatory (83 ± 9 µg mL-1) and cytotoxic activities, thus presenting slight toxicity using a non-tumor cell line. Regarding the phenolic profile, the ethyl acetate fraction presented twelve flavonoids, with morelloflavone-7''-O-glucoside (52.1 ± 0.4 mg g-1) and gardinia biflavonoid 2a glucoside (27.5 ± 0.2 mg g-1) being the major compounds identified. These results indicate that leaves of G. brasiliensis might be a potential source of natural biomolecules for pharmaceutical and medicinal applications.
Assuntos
Garcinia/química , Compostos Fitoquímicos/análise , Folhas de Planta/química , Anti-Infecciosos/análise , Antioxidantes/análise , Brasil , Candida albicans/efeitos dos fármacos , Flavonoides/análise , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Humanos , Células MCF-7 , Fenóis/análise , Extratos Vegetais/análiseRESUMO
O presente trabalho teve o objetivo de avaliar a qualidade microbiológica de forma qualitativa, e quantitativamente as propriedades físico-químicas das águas minerais comercializadas na Região do Vale do Jamari Rondônia Brasil, composta hoje por nove municípios. Para as análises foram utilizadas seis marcas diferentes e de cada uma delas, três lotes distintos. Ao se tratar das análises da qualidade microbiológica foi utilizado o método rápido de Colitag em 100 ml de água mineral, identificando a presença de Coliformes totais e Coliformes termotolerantes (Escherichia coli). As análises físico-químicas avaliaram os parâmetros de pH, condutividade elétrica, temperatura, turbidez e a concentração de flúor presente. Os resultados apresentaram contaminação com bactérias do grupo coliformes totais em todos os lotes analisados, e em alguns a presença de Escherichia coli. Em relação às análises físico-químicas a maioria das marcas apresentou resultados próximos em comparação às informações contidas nos rótulos.
This study aimed to evaluate the microbiological quality qualitatively and quantitatively the physicochemical properties of mineral waters marketed in Vale do Jamari - Rondônia - Brazil, composed of nine counties today. For the analysis we used six different brands and each of three different lots. When dealing with the analysis of microbiological method was used for rapid Colitag in 100 ml of mineral water, identifying the presence of total coliforms and thermotolerant coliforms (Escherichia coli). The physico-chemical parameters evaluated for pH, electrical conductivity, temperature, turbidity and fluoride concentration. The results were contaminated with total coliform bacteria in all analyzed batches, and in some cases the presence of Escherichia coli. Regarding physicochemical analyzes most brands had similar results compared to the information on the labels.
